Hyderabad, Jun 2 : A simple and fast method of Dry Swab-based Direct RT-PCR developed by CSIR’s constituent lab Centre for Cellular and Molecular Biology (CCMB), Hyderabad, can help easily scale up the coronavirus testing by 2 to 3 fold without any new investment, according to CCMB.
This method, approved by ICMR based on their independent validation, is a simple variation of the existing gold standard RT-PCR method, CCMB said in a release here Today.
Meril , an Indian origin, global medical device company, would manufacture commercial kits for CCMB’s Dry Swab Direct RT-PCR tests.
Meril is currently equipped to manufacture 2 crore kits a month and each kit suffices for 100 tests.Using these kits, each test will cost between Rs 45-60.
CSIR-CCMB Director Dr Vinay Nandicoori, said “In this method, one can simply break open the cells by using a buffer solution and use them for RT-PCR directly. There is no need for separate steps of RNA extraction and purification.”
CSIR-CCMB Advisor Dr Rakesh Mishra, said “RNA extraction, even with automation, takes 4 hours for roughly 500 samples. Viral Transport Medium (VTM) and RNA extraction both add a significant burden on money and time required for mass testing for coronavirus. We believe this method has the potential of bringing the costs and time of testing by 40-50 percent in all kinds of settings.”
CSIR-CCMB has tied up with Meril Diagnostics, for commercially scaling up the Dry Swab-based tests across diagnostic labs in the country.
Meril’s Extraction-free Dry Swab Kit can be used to isolate the viral RNA from dry swab samples. These isolates can then be directly used for RT-PCR.
“We are the first company to commercially launch the dry swab kit. We truly believe the use case for the dry swab collection sample technique is very large. It is imperative in these times to get a quick RT-PCR result and this method helps in doing so by eliminating the lengthy RNA extraction process. We believe this will help scale testing in India, and help government and private labs in reducing their turnaround time. This is the need of the hour”, said Sanjeev Bhatt, Vice President – Corporate Strategy, Meril.(UNI)